N1 Single Cell Dispenser
Sorting for 96 or 384-well plates

High Viability

Our single cell dispenser is ideal for a multitude of applications including single cell cloning and works seamlessly with our WOLF cell sorter microfluidic device for easy single cell isolation, single cell analysis, and cell line development. The gentle sorting mechanism and single cell manipulation results in improved viability of  each individual cell and higher outgrowth for a healthier cell population. Reduced stress via gentle microfluidic cell sorting (low throughput) process also avoids potential adverse gene expression or karyotype changes experienced with a traditional cell sorter.

Sterile Microfluidics

Disposable microfluidic cartridge, no biohazardous aerosol exposure, no sample to sample contamination within the microfluidic based cell sorting platform.

Fluidic Flexibility

The WOLF uses a small fraction of the sheath fluid used by traditional sorters, allowing users to use growth media as sheath. Using growth media as sheath greatly improves cell viability for cell isolation, FACS (fluorescence activated cell sorting), and overall flow cytometry analysis.

Single Cell Efficiency

The 5 parameters of the WOLF and N1 provide higher rates of singlet detection compared to cell printers or limiting dilution.

Easy-to-use

Designed from a user’s perspective, the software interface is easy-to-use while featuring powerful analytical features. Step-by-step guides help users get from sample to sorted cells quickly.

Small Footprint

Compared to a traditional flow cytometry lab setup, the robust, portable, sterile, and safe system can be used in any lab without the need for dedicated hoods, venting, or personnel. Light enough to be moved and shared.

A module for the WOLF Cell Sorter to sort and dispense into 96-well or 384-well plates.

Applications requiring the isolation, sorting and dispensing of single cells, such as B-cell identification in antibody discovery, and monoclonal CHO cell line development, are now easier with the N1® Single Cell Dispenser. Additionally, single-cell genomics assays, where sample preparation is a key step for quality data generation, no longer have to be heroic academic exercises when employing this WOLF® accessory.

Users can select one population of cells for single cell deposition into 96- or 384-well plates or choose two different populations of cells for bulk cell sorting. RNA Sequencing applications operators can use the N1 96- or 384-well plate dispenser to isolate cells into PCR plates.

In the case of cell line development, users can use the N1 384-well plate dispenser to isolate cells for monoclonal outgrowth.  

Each well of a 96- or 384-well plate can receive anywhere from 1 to 100 cells as they are sorted from the sample. Or users can have the cell dispenser operate from a pure population.

The N1 Single Cell Dispenser is seamlessly integrated into the intuitive software. Select single-cell from the dropdown menu, map out your plate, and hit go. The WOLF cell sorter and dispenser work together to deliver plated cells in a compact and affordable format. After sorting, data from each cell is indexed well-by-well, allowing for confirmation of the plated cell.

Forget the traditional old school flow cytometer/cell printers/cell plating techniques that damage target cells with high pressure and high throughput devices. Instead, start increasing your efficiency by adopting gentle microfluidic technology into your lab. For accurate cell deposition and results, NanoCellect has you and your lab covered.